Sodium ions (Na+) are key regulators of molecular events in many cellular processes. yet the dynamics of this ion remain poorly defined. Developing approaches to identify and characterise Na+ microenvironments will enable more detailed elucidation of the mechanisms of signal transduction. Here we report the application of Spectral Phasor analysis to the Na+ fluorophore. https://storeanimalwiz.shop/product-category/filter-cartridges/